| Predicted Species Reactivity | Rat |
|---|---|
| Product Format | Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | WB |
| Additional Information | Packaging : 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
| Reconstitution and Storage | Store at -20C in undiluted aliquots; stable for up to one year after date of receipt. Avoid freeze/thaw cycles. |
| Replacement Item | This antibody may replace item sc-113925 from Santa Cruz Biotechnology. |
| Immunogen | Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat TrkB. |
| Purification | Prepared from rabbit serum by affinity purification on a column made with the C-terminal peptide used as antigen. |
| Predicted Homology Based on Immunogen Sequence | Rat |
| Datasheets/Manuals | Printable datasheet for OAPC00072 |
| Specificity | Specific for the ~93 kDa TrkB protein in Western blots. |
| Application Info | The antibody has been directly tested for reactivity in rat.Western blot of rat synaptic membrane preparation showing specific immunolabeling of the ~ 93k TrkB protein. |
| Target Reference | Soppet D, Escandon E, Maragos J, Middlemas DS, Reid SW, Blair J, Burton LE, Stanton BR, Kaplan DR, Hunter T, Nikolics K, Parada LF (1991) The neurotrophic factors brain-derived neurotrophic factor and neurotrophin-3 are ligands for the trkB tyrosine kinase receptor. Cell. May 31;65(5):895-903.Klein R, Nanduri V, Jing SA, Lamballe F, Tapley P, Bryant S, Cordon-Cardo C, Jones KR, Reichardt LF, Barbacid M (1991) The trkB tyrosine protein kinase is a receptor for brain-derived neurotrophic factor and neurotrophin-3. Cell. Jul 26;66(2):395-403.Ferrer I, MarÃn C, Rey MJ, Ribalta T, Goutan E, Blanco R, Tolosa E, Martà E (1999) BDNF and full-length and truncated TrkB expression in Alzheimer disease. Implications in therapeutic strategies. J Neuropathol Exp Neurol. Jul;58(7):729-39.Chen Z, Simmons MS, Perry RT, Wiener HW, Harrell LE, Go RC. (2008) Genetic association of neurotrophic tyrosine kinase receptor type 2 (NTRK2) With Alzheimer"s disease. Am J Med Genet B Neuropsychiatr Genet. Apr 5;147(3):363-9. |
| Gene Symbol | Ntrk2 |
|---|---|
| NCBI Gene Id | 25054 |
| Protein Name | BDNF/NT-3 growth factors receptor |
| Description of Target | Tropomyosin-receptor-kinase (TRK) receptors are members of the receptor tyrosine kinase family and include TrkA, TrkB and TrkC. Each of these receptor types has different binding affinities to the neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin 3 (NT-3). TrkB has the highest affinity to binding BDNF which plays critical roles in the function and survival of neurons in the CNS (Soppet et al., 1991; Klein et al., 1991). Additionally, alterations in expression of TrkB have been associated with Alzheimer"s disease (Ferrer et al., 1999; Chen et al., 2008). |
| Swissprot Id | Q63604 |
| Protein Accession # | NP_001156640.1 |
| Molecular Weight | ~93kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express Ntrk2.  |
| RNA Seq | Find tissues and cell lines supported by DNA array analysis to express Ntrk2.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
What is the species homology for "Anti-TrkB (OAPC00072)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Rat".
How long will it take to receive "Anti-TrkB (OAPC00072)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
What buffer format is "Anti-TrkB (OAPC00072)" provided in?
This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "Anti-TrkB (OAPC00072)"?
This target may also be called "" in publications.
What is the shipping cost for "Anti-TrkB (OAPC00072)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "Anti-TrkB (OAPC00072)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "Anti-TrkB (OAPC00072)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~93kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "Anti-TrkB (OAPC00072)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "NTRK2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "NTRK2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "NTRK2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "NTRK2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "NTRK2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "NTRK2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
