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avivasysbio/Anti-GABA Alpha Receptor, Delta -Subunit, N-Terminus (OAPC00135)/100 ul/OAPC00135
| Predicted Species Reactivity | Mouse, Rat |
|---|---|
| Product Format | Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | IHC |
| Additional Information | Packaging : 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
| Reconstitution and Storage | For long term storage -20C is recommended. Stable at -20C for at least 1 year. |
| Immunogen | Fusion protein from the N-terminus of the delta -subunit of rat GABAA receptor. |
| Purification | Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled. |
| Predicted Homology Based on Immunogen Sequence | Mouse; Rat |
| Datasheets/Manuals | Printable datasheet for OAPC00135 |
| Specificity | Specific for the delta -subunit of the GABAA receptor. |
| Application Info | The antibody has been directly tested for reactivity in immunohistochemistry with rat and mouse tissue.Immunohistochemical staining of rat hippocampus showing specific labeling of the GABA A receptor, delta subunit. |
| Target Reference | Korpi ER, Mihalek RM, Sinkkonen ST, Hauer B, Hevers W, Homanics GE, Sieghart W, Luddens H (2002) Altered receptor subtypes in the forebrain of GABAA receptor delta -subunit-deficient mice: recruitment of gamma 2-subunits. Neurosci 109:733-743.McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha 1-subtype. Nature Neurosci 3:587-592.Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing alpha 1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199.Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma 2-subunit. Biochem Pharmacol 68:1621-1629.Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.Pöltl A, Hauer B, Fuchs K, Tretter V, Sieghart W (2003) Subunit composition and quantitative importance of GABAA receptor subtypes in the cerebellum of mouse and rat. J Neurochem 87:1444-1455.Wallner M, Hanchar HJ, Olsen RW (2003) From The Cover: Ethanol enhances alpha 4 beta 3 delta and alpha 6 beta 3 delta gamma -aminobutyric acid type A receptors at low concentrations known to affect humans. Proc Natl Acad Sci (USA) 100:15218-15223.Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdellès B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA, Wafford KA (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653.Wohlfarth KM, Bianchi MT, Macdonald RL (2002) Enhanced Neurosteroid Potentiation of Ternary GABAA Receptors Containing the delta -Subunit. J Neurosci 22:1541-1549. |
| Gene Symbol | GABRD |
|---|---|
| NCBI Gene Id | 29689 |
| Protein Name | Gamma-aminobutyric acid receptor subunit delta |
| Description of Target | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alpha s, four beta s and four gamma s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). The various effects of the benzodiazepines in brain may be mediated via different alpha -subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). More recently there have been a number of studies demonstrating that the delta -subunit of the receptor may affect subunit assembly (Korpi et al., 2002) and may also confer differential sensitivity to neurosteroids and to ethanol (Wallner et al., 2003; Wohlfarth et al., 2002). |
| Swissprot Id | P18506 |
| Protein Accession # | NP_058985.1 |
| Molecular Weight | ~52kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express GABRD.  |
| RNA Seq | Find tissues and cell lines supported by DNA array analysis to express GABRD.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
What is the species homology for "Anti-GABA Alpha Receptor, Delta -Subunit, N-Terminus (OAPC00135)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse, Rat".
How long will it take to receive "Anti-GABA Alpha Receptor, Delta -Subunit, N-Terminus (OAPC00135)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
What buffer format is "Anti-GABA Alpha Receptor, Delta -Subunit, N-Terminus (OAPC00135)" provided in?
This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "Anti-GABA Alpha Receptor, Delta -Subunit, N-Terminus (OAPC00135)"?
This target may also be called "" in publications.
What is the shipping cost for "Anti-GABA Alpha Receptor, Delta -Subunit, N-Terminus (OAPC00135)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "Anti-GABA Alpha Receptor, Delta -Subunit, N-Terminus (OAPC00135)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "Anti-GABA Alpha Receptor, Delta -Subunit, N-Terminus (OAPC00135)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~52kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "Anti-GABA Alpha Receptor, Delta -Subunit, N-Terminus (OAPC00135)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "GABRD"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "GABRD"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "GABRD"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "GABRD"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "GABRD"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "GABRD"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
