| Predicted Species Reactivity | Cow, Dog, Human, Mouse, Rat |
|---|---|
| Product Format | Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | WB |
| Additional Information | Packaging : 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
| Reconstitution and Storage | Store at -20C; stable for at least one year. |
| Replacement Item | This antibody may replace item sc-172182 from Santa Cruz Biotechnology. |
| Immunogen | Peptide corresponding to amino acid residues from the C-terminal region of rat GRASP, conjugated to keyhole limpet hemocyanin (KLH). |
| Purification | Prepared from rabbit serum by affinity purification via chromatography on an affinity column prepared with the C-terminal peptide used as antigen. |
| Predicted Homology Based on Immunogen Sequence | Cow; Dog; Human; Mouse; Rat |
| Datasheets/Manuals | Printable datasheet for OAPC00116 |
| Specificity | Specific for the ~95k GRASP protein. |
| Application Info | The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.Western blot of rat cerebellar lysate showing the specific immunolabeling of the ~95k GRASP protein. |
| Target Reference | Scannevin RH, Huganir RL (2000) Postsynaptic organization and regulation of excitatory synapses. Nat Rev Neurosci 1:133-141.Ye B, Liao DZ, Zhang XQ, Zhang PS, Dong HL, Huganir RL (2000) GRASP-1: A neuronal RasGEF associated with the AMPA receptor/GRIP complex. Neuron 26:603-617. |
| Gene Symbol | GRIPAP1 |
|---|---|
| NCBI Gene Id | 116493 |
| Protein Name | GRIP1-associated protein 1 |
| Description of Target | PDZ domain-containing proteins, such as PSD-95 and GRIP are thought to play key roles in glutamate receptor plasticity. GRIP-associated proteins (GRASPs) that bind to distinct PDZ domains within GRIP also play key roles in regulation of glutamate receptor function. GRASP-1 is a neuronal rasGEF associated with GRIP and AMPA receptors in vivo (Scannevin and Huganir, 2000). Recent work suggests that GRASP-1 may regulate neuronal ras signaling and contribute to the regulation of AMPA receptor distribution by NMDA receptor activity (Ye et al., 2000). |
| Swissprot Id | Q9JHZ4 |
| Protein Accession # | NP_446259.1 |
| Molecular Weight | ~95kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express GRIPAP1.  |
| RNA Seq | Find tissues and cell lines supported by DNA array analysis to express GRIPAP1.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
What is the species homology for "Anti-GRASP (OAPC00116)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Cow, Dog, Human, Mouse, Rat".
How long will it take to receive "Anti-GRASP (OAPC00116)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
What buffer format is "Anti-GRASP (OAPC00116)" provided in?
This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "Anti-GRASP (OAPC00116)"?
This target may also be called "" in publications.
What is the shipping cost for "Anti-GRASP (OAPC00116)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "Anti-GRASP (OAPC00116)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "Anti-GRASP (OAPC00116)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~95kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "Anti-GRASP (OAPC00116)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "GRIPAP1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "GRIPAP1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "GRIPAP1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "GRIPAP1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "GRIPAP1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "GRIPAP1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
