| Predicted Species Reactivity | Human, Mouse, Rat |
|---|---|
| Product Format | Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | WB, IHC |
| Additional Information | Packaging : 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
| Reconstitution and Storage | Store at -20C; stable for at least one year. |
| Replacement Item | This antibody may replace item sc-125157 from Santa Cruz Biotechnology. |
| Immunogen | peptide from the human collagen I alpha 1 propeptide sequence. |
| Purification | Affinity purified |
| Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
| Datasheets/Manuals | Printable datasheet for OAPC00094 |
| Specificity | Specific for the propeptide portion of the ~180 kDa collagen I alpha 1 polypeptide in human lung fibroblast extract. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized. |
| Application Info | The antibody has been directly tested for reactivity in human, mouse and rat. Based on the homology of the antigen this antibody is expected to recognize the collagen I alpha 1 polypeptide in most, if not all, species of mammals, birds, amphibians, and fishes.Western blot of rat lung lysate showing specific immunolabeling of the ~180 k collagen 1IHC of fibrotic mouse lung tissue showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized. |
| Target Reference | Byers PH (1989) Inherited disorders of collagen gene structure and expression. Am J Med Genet. 34(1):72-80.Cabral WA, Makareeva E, Colige A, Letocha AD, Ty JM, Yeowell HN, Pals G, Leikin S, Marini JC. (2005) Mutations near amino end of alpha1(I) collagen cause combined osteogenesis imperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing. J Biol Chem. 2005 May 13;280(19):19259-69. |
| Gene Symbol | COL1A1 |
|---|---|
| NCBI Gene Id | 1277 |
| Protein Name | Collagen alpha-1(I) chain |
| Description of Target | Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the must abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005). |
| Swissprot Id | P02452 |
| Protein Accession # | NP_000079.2 |
| Molecular Weight | ~180kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express COL1A1.  |
| RNA Seq | Find tissues and cell lines supported by DNA array analysis to express COL1A1.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
What is the species homology for "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
How long will it take to receive "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
What buffer format is "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)" provided in?
This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
This target may also be called "" in publications.
What is the shipping cost for "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~180kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "Anti-Collagen I alpha 1 ProPeptide Sequence (OAPC00094)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "COL1A1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "COL1A1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "COL1A1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "COL1A1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "COL1A1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "COL1A1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
