| Predicted Species Reactivity | Human, Mouse, Rat, Chicken |
|---|---|
| Product Format | Liquid. PBS with no preservative. Adequate amount of material to conduct 10 mini Western Blots. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | WB, IHC |
| Reconstitution and Storage | For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable. Stable for a minimum of 1 year at -20°C. Avoid freeze/thaw cycles. |
| Replacement Item | This antibody may replace item sc-398631 from Santa Cruz Biotechnology. |
| Immunogen | Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminal region of mouse periostin which is comprised of six small alternatively-spliced exons. |
| Purification | Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled. |
| Concentration | 0.25 mg/ml |
| Datasheets/Manuals | Printable datasheet for OAPC00052 |
| Specificity | Specific for the ~93 kDa periostin doublet in mouse lung extract. Consistent with the fact that this antibody is made against an alternatively spliced region of periostin, this antibody recognizes only the largest two of the three forms of periostin recognized on Western blots by the Pan periostin antibody (Cat. # OAPC00053) and also shows a distinctive staining pattern by immunohistochemistry (data not shown). The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). |
| Intended Use | For research use only. |
| Application Info | IHC 1:1000WB 1:1000 |
| Target Reference | Kruzynska-Frejtag A, Wang J, Rogers R, Krug E, Hoffman S, Markwald RR, Conway SJ. (2004)Periostin is expressed within the developing teeth at the sites of epithelial-mesenchymalinteraction. Develop Dynamics, 229:857-868.Norris RA, Borq TK, Butcher JT, Baudino TA, Banerjee I, Markwald RR (2008) Neonatal and adult cardiovascular pathophysiological remodeling and repair: developmental role of periostin. Ann NY Acad Sci. 1123:30-40.Norris RA, Damon B, Mironov V, Kasyanov V, Ramamurthi A, Moreno-Rodriguez R, Trusk T,Potts JD, Goodwin RL, Davis J, Hoffman S, Wen X, Sugi Y, Kern CB, Mjaatvedt CH, Turner DK,Oka T, Conway SJ, Molkentin JD, Forgacs G, Markwald RR. (2007) Periostin regulates collagenfibrillogenesis and the biomechanical properties of connective tissues. J Cell Biochem.101(3):695-711. |
| Publications | Midgett, M; López, CS; David, L; Maloyan, A; Rugonyi, S; Increased Hemodynamic Load in Early Embryonic Stages Alters Endocardial to Mesenchymal Transition. 8, 56 (2017). WB, IHC, Human, Mouse, Rat, Chicken 28228731 |
| Gene Symbol | POSTN |
|---|---|
| NCBI Gene Id | 50706 |
| Protein Name | Periostin |
| Description of Target | Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has since been demonstrated to be important in heart valve development and myocardial infarction because it promotes collagen fibrogenesis, inhibits differentiation of progenitor cells into cardiomyocytes and is essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008). |
| Swissprot Id | Q62009 |
| Protein Accession # | NP_001185694.1 |
| Molecular Weight | ~93kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express POSTN.  |
| RNA Seq | Find tissues and cell lines supported by DNA array analysis to express POSTN.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
What is the species homology for "Anti-Periostin, C-terminal (OAPC00052)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Chicken".
How long will it take to receive "Anti-Periostin, C-terminal (OAPC00052)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
What buffer format is "Anti-Periostin, C-terminal (OAPC00052)" provided in?
This item is provided in "Liquid. PBS with no preservative. Adequate amount of material to conduct 10 mini Western Blots.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "Anti-Periostin, C-terminal (OAPC00052)"?
This target may also be called "" in publications.
What is the shipping cost for "Anti-Periostin, C-terminal (OAPC00052)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "Anti-Periostin, C-terminal (OAPC00052)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "Anti-Periostin, C-terminal (OAPC00052)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~93kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "Anti-Periostin, C-terminal (OAPC00052)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "POSTN"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "POSTN"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "POSTN"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "POSTN"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "POSTN"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "POSTN"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
