| Tested Species Reactivity | Human, Mouse |
|---|---|
| Predicted Species Reactivity | Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish |
| Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | IF, IP, WB, IHC |
| Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Replacement Item | This antibody may replace item sc-120760 from Santa Cruz Biotechnology. |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human HES1 |
| Purification | Protein A purified |
| Predicted Homology Based on Immunogen Sequence | Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Complete computational species homology data | Anti-HES1 (ARP32372_T100) |
| Peptide Sequence | Synthetic peptide located within the following region: NTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSSR |
| Concentration | Batch dependent within range: 100 ul at 0.5 - 1 mg/ml |
| Blocking Peptide | For anti-HES1 (ARP32372_T100) antibody is Catalog # AAP32372 (Previous Catalog # AAPP03361) |
| Datasheets/Manuals | Printable datasheet for anti-HES1 (ARP32372_T100) antibody |
| Sample Type Confirmation | HES1 is strongly supported by BioGPS gene expression data to be expressed in Raji |
| Target Reference | Hoebeke,I., et al., (2006) Blood 107 (7), 2879-2881 |
| Publications | Asnaghi, L. et al. Hypoxia Promotes Uveal Melanoma Invasion through Enhanced Notch and MAPK Activation. PLoS One 9, e105372 (2014). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 25166211 Asnaghi, L. et al. Notch signaling promotes growth and invasion in uveal melanoma. Clin. Cancer Res. 18, 654-65 (2012). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 22228632 Bennani-Baiti, I. M. et al. Notch signalling is off and is uncoupled from HES1 expression in Ewing’s sarcoma. J. Pathol. 225, 353-63 (2011). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 21984123 Chu, Q., Liu, L. & Wang, W. Overexpression of hCLP46 enhances Notch activation and regulates cell proliferation in a cell type-dependent manner. Cell Prolif. 46, 254-62 (2013). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 23692084 Guiu, J. et al. Hes repressors are essential regulators of hematopoietic stem cell development downstream of Notch signaling. J. Exp. Med. 210, 71-84 (2013). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 23267012 Ma, L. et al. Atractylenolide I-mediated Notch pathway inhibition attenuates gastric cancer stem cell traits. Biochem. Biophys. Res. Commun. 450, 353-9 (2014). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 24944018 Ma, W. et al. hCLP46 regulates U937 cell proliferation via Notch signaling pathway. Biochem. Biophys. Res. Commun. 408, 84-8 (2011). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 21458412 Meng, S; Su, Z; Liu, Z; Wang, N; Wang, Z; Rac1 contributes to cerebral ischemia reperfusion-induced injury in mice by regulation of Notch2. 306, 100-14 (2015). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 26299339 Natsumeda, M; Maitani, K; Liu, Y; Miyahara, H; Kaur, H; Chu, Q; Zhang, H; Kahlert, UD; Eberhart, CG; Targeting Notch Signaling and Autophagy Increases Cytotoxicity in Glioblastoma Neurospheres. 26, 713-723 (2016). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 26613556 Schreck, K. C. et al. The Notch target Hes1 directly modulates Gli1 expression and Hedgehog signaling: a potential mechanism of therapeutic resistance. Clin. Cancer Res. 16, 6060-70 (2010). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 21169257 Tilley, A. E. et al. Down-regulation of the notch pathway in human airway epithelium in association with smoking and chronic obstructive pulmonary disease. Am. J. Respir. Crit. Care Med. 179, 457-66 (2009). IF, IP, WB, IHC, Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 19106307 |
| Gene Symbol | HES1 |
|---|---|
| Official Gene Full Name | Hairy and enhancer of split 1, (Drosophila) |
| Alias Symbols | HHL, HRY, HES-1, bHLHb39 |
| NCBI Gene Id | 3280 |
| Protein Name | Transcription factor HES-1 |
| Description of Target | HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. |
| Swissprot Id | Q14469 |
| Protein Accession # | NP_005515 |
| Nucleotide Accession # | NM_005524 |
| Protein Size (# AA) | 280 |
| Molecular Weight | 30kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express HES1.  |
| RNA Seq | Find tissues and cell lines supported by RNA-seq analysis to express HES1.  |
| Protein Interactions | STAT3; JAK2; APH1A; LTBR; ASGR2; TLE1; ELAVL1; PARP1; UBC; NUDT3; HDAC6; YWHAB; PTK2; NR4A1; HMGCL; GAPDH; FHL1; CSNK1E; APCS; FANCE; FANCC; FANCA; FANCL; SPTAN1; HSP90AA1; HSPA4; FANCG; FANCF; HDAC1; RUNX2; HES6; ID4; ID1; SIRT1; UBQLN1; HEY2; ID2; ID3; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
- Reviewed Data:
- Customer Reviews for HES1 Antibody (ARP32372_T100) tested with LS174T cells in Immunofluorescence
- Product Protocols: HES1 antibody tested with Human Raji Cells (ARP32372_T100)
- Product Review: HES1 antibody - N-terminal region (ARP32372_T100) in Mouse embryonic stem cells using Western Blot
- Product Review: HES1 antibody-N-terminal region (ARP32372_T100) in K562 cells using IP
What is the species homology for "HES1 Antibody - N-terminal region (ARP32372_T100)"?
The tested species reactivity for this item is "Human, Mouse". This antibody is predicted to have homology to "Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish".
How long will it take to receive "HES1 Antibody - N-terminal region (ARP32372_T100)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
What buffer format is "HES1 Antibody - N-terminal region (ARP32372_T100)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "HES1 Antibody - N-terminal region (ARP32372_T100)"?
This target may also be called "HHL, HRY, HES-1, bHLHb39" in publications.
What is the shipping cost for "HES1 Antibody - N-terminal region (ARP32372_T100)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "HES1 Antibody - N-terminal region (ARP32372_T100)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "HES1 Antibody - N-terminal region (ARP32372_T100)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "30kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "HES1 Antibody - N-terminal region (ARP32372_T100)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "HES1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "HES1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "HES1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "HES1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "HES1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "HES1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
