| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat |
| Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | WB |
| Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Replacement Item | This antibody may replace item sc-113676 from Santa Cruz Biotechnology. |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human CLK1 |
| Purification | Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 85% |
| Complete computational species homology data | Anti-CLK1 (ARP52021_P050) |
| Peptide Sequence | Synthetic peptide located within the following region: HSSAQENKRCKYNHSKMCDSHYLESRSINEKDYHSRRYIDEYRNDYTQGC |
| Concentration | Batch dependent within range: 100 ul at 0.5 - 1 mg/ml |
| Blocking Peptide | For anti-CLK1 (ARP52021_P050) antibody is Catalog # AAP52021 (Previous Catalog # AAPP44291) |
| Datasheets/Manuals | Printable datasheet for anti-CLK1 (ARP52021_P050) antibody |
| Sample Type Confirmation | CLK1 is supported by BioGPS gene expression data to be expressed in HEK293T |
| Publications | Aubol, BE; Wu, G; Keshwani, MM; Movassat, M; Fattet, L; Hertel, KJ; Fu, XD; Adams, JA; Release of SR Proteins from CLK1 by SRPK1: A Symbiotic Kinase System for Phosphorylation Control of Pre-mRNA Splicing. 63, 218-28 (2016). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 27397683 Eisenreich, A. et al. Regulation of pro-angiogenic tissue factor expression in hypoxia-induced human lung cancer cells. Oncol. Rep. 30, 462-70 (2013). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 23604472 |
| Gene Symbol | CLK1 |
|---|---|
| Official Gene Full Name | CDC-like kinase 1 |
| Alias Symbols | CLK, CLK/STY, STY |
| NCBI Gene Id | 1195 |
| Protein Name | Dual specificity protein kinase CLK1 |
| Description of Target | This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Swissprot Id | P49759 |
| Protein Accession # | NP_004062 |
| Nucleotide Accession # | NM_004071 |
| Protein Size (# AA) | 484 |
| Molecular Weight | 57kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express CLK1.  |
| RNA Seq | Find tissues and cell lines supported by RNA-seq analysis to express CLK1.  |
| Protein Interactions | KRTAP10-7; PDE9A; PRMT5; SRPK2; SRPK1; CLK1; BAG3; PIAS4; ISG15; UBC; YWHAG; LUC7L3; PPIG; SRSF6; PRPF4; GOLM1; UBL5; SRSF1; PTPN1; MBP; BCLAF1; NCOR1; SRSF3; SAFB; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
- Reviewed Data:
- Product Review: CLK1 antibody - N-terminal region (ARP52021_P050) with human microvascular endothelial cells (HMEC-1)for Western Blot
- Product Protocols: CLK1 antibody tested with Human 293T Cells (ARP52021_P050)
What is the species homology for "CLK1 Antibody - N-terminal region (ARP52021_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat".
How long will it take to receive "CLK1 Antibody - N-terminal region (ARP52021_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
What buffer format is "CLK1 Antibody - N-terminal region (ARP52021_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "CLK1 Antibody - N-terminal region (ARP52021_P050)"?
This target may also be called "CLK, CLK/STY, STY" in publications.
What is the shipping cost for "CLK1 Antibody - N-terminal region (ARP52021_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "CLK1 Antibody - N-terminal region (ARP52021_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "CLK1 Antibody - N-terminal region (ARP52021_P050)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "57kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "CLK1 Antibody - N-terminal region (ARP52021_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "CLK1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "CLK1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "CLK1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "CLK1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "CLK1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "CLK1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
