| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Liquid. PBS with 0.09% (W/V) sodium azide. |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Host | Rabbit |
| Application | WB, FC, IHC-P |
| Additional Information | Function: Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3"-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. Cellular Location: Nucleus. Nucleus speckle. |
| Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Replacement Item | This antibody may replace item sc-19663 from Santa Cruz Biotechnology. |
| Immunogen | This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-125 amino acids from the Central region of human U2AF1. |
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Predicted Homology Based on Immunogen Sequence | Cow; Mouse |
| Datasheets/Manuals | Printable datasheet for anti-U2AF1 antibody - OAAB01511 |
| Intended Use | For research use only and not for use in diagnostic or therapeutic procedures. |
| Application Info | WB: 1:1000IHC-P: 1:50~100FC: 1:10~50 |
| Other Applications Image 1 Data | U2AF1 Antibody (Center Y114) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
| Gene Symbol | U2AF1 |
|---|---|
| Alias Symbols | Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP |
| NCBI Gene Id | 7307 |
| Protein Name | Splicing factor U2AF 35 kDa subunit |
| Description of Target | U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. |
| Swissprot Id | Q01081 |
| Protein Accession # | NP_001020374.1 |
| Molecular Weight | 27872 Da (35 kDa subunit) Da |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express U2AF1.  |
| RNA Seq | Find tissues and cell lines supported by RNA-seq analysis to express U2AF1.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
- Reviewed Data:
- Product Protocol: U2AF1 antibody tested with human Ramos by western blot (OAAB01511)
- Product Protocol: U2AF1 antibody tested with rabbit U2AF1 by western blot (OAAB01511)
- Product Protocol: U2AF1 antibody tested with human lung carcinoma in immunohistochemistry (IHC) (OAAB01511)
What is the species homology for "U2AF1 Antibody (OAAB01511)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
How long will it take to receive "U2AF1 Antibody (OAAB01511)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
What buffer format is "U2AF1 Antibody (OAAB01511)" provided in?
This item is provided in "Liquid. PBS with 0.09% (W/V) sodium azide.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "U2AF1 Antibody (OAAB01511)"?
This target may also be called "Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP" in publications.
What is the shipping cost for "U2AF1 Antibody (OAAB01511)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "U2AF1 Antibody (OAAB01511)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "U2AF1 Antibody (OAAB01511)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "27872 Da (35 kDa subunit) Da".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "U2AF1 Antibody (OAAB01511)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "U2AF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "U2AF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "U2AF1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "U2AF1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "U2AF1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "U2AF1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
