| Predicted Species Reactivity | Human, Mouse, Rat |
|---|---|
| Product Format | Liquid |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | WB, IHC |
| Reconstitution and Storage | Store at -20C. Avoid repeated freeze/thaw cycles. |
| Immunogen | A phospho-Serpecific peptide corresponding-Thro residues-Serurrounding-Ser259 of human RAF1 |
| Purification | Affinity purified against immunogen |
| Datasheets/Manuals | Printable datasheet for anti-RAF1 (OAAN03076) |
| Preservative | 0.02% Sodium Azide |
| Target Post-Translational Modification | Phospho-Ser259 |
| Application Info | WB: 1:500 - 1:2,000. IHC: 1:50 - 1:100 |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Gene Symbol | RAF1 |
|---|---|
| Official Gene Full Name | Raf-1 proto-oncogene, serine/threonine kinase |
| Alias Symbols | NS5, CRAF, Raf-1, c-Raf, NS5, CRAF, Raf-1, c-Raf, CMD1NN, Raf-1 proto-oncogene, serine/threonine kinase, RAF proto-oncogene serine/threonine-protein kinase, RAF1 |
| NCBI Gene Id | 5894 |
| Protein Name | RAF proto-oncogene serine/threonine-protein kinase |
| Description of Target | This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. |
| Swissprot Id | P04049 |
| Protein Accession # | NP_002871.1 |
| Nucleotide Accession # | NM_002880.3 |
| Molecular Weight | 73 kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express RAF1.  |
| RNA Seq | Find tissues and cell lines supported by RNA-seq analysis to express RAF1.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
What is the species homology for "RAF1 Antibody (Phospho-Ser259) (OAAN03076)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
How long will it take to receive "RAF1 Antibody (Phospho-Ser259) (OAAN03076)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
What buffer format is "RAF1 Antibody (Phospho-Ser259) (OAAN03076)" provided in?
This item is provided in "Liquid".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "RAF1 Antibody (Phospho-Ser259) (OAAN03076)"?
This target may also be called "NS5, CRAF, Raf-1, c-Raf, NS5, CRAF, Raf-1, c-Raf, CMD1NN, Raf-1 proto-oncogene, serine/threonine kinase, RAF proto-oncogene serine/threonine-protein kinase, RAF1" in publications.
What is the shipping cost for "RAF1 Antibody (Phospho-Ser259) (OAAN03076)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "RAF1 Antibody (Phospho-Ser259) (OAAN03076)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "RAF1 Antibody (Phospho-Ser259) (OAAN03076)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "73 kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "RAF1 Antibody (Phospho-Ser259) (OAAN03076)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.
What are positive controls for "RAF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "RAF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "RAF1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "RAF1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "RAF1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "RAF1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
