| Predicted Species Reactivity | Human, Mouse, Rat |
|---|---|
| Product Format | Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | WB |
| Additional Information | Modification Sites: H:S52 M:S52 R:S52Target modification: Phospho |
| Reconstitution and Storage | Store at -20C/1 year |
| Replacement Item | This antibody may replace item sc-11386 from Santa Cruz Biotechnology. |
| Immunogen | The antiserum was produced against synthesized peptide derived between 21-70 amino acids from the Internal region of human eIF2 alpha around the phosphorylation site of Ser51. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Datasheets/Manuals | Printable datasheet for OAAF00311 |
| Specificity | eIF2 alpha (phospho-Ser51) antibody detects endogenous levels of eIF2 alpha only when phosphorylated at serine 51 |
| Dilution | The optimal working dilution should be determined by the end user. |
| Application Info | WB: 1:500~1:1000ELISA: 1:10,000 |
| Target Reference | Xavier Saelens, et,al. (2001) J. Biol. Chem; 276: 41620-41628.Hiroyuki Kubota,et,al.(2003) J. Biol. Chem; 278: 20457-20460.Shijian Chu, et,al. (2006) Am J Physiol Lung Cell Mol Physiol; 291: L983-L992.Eileen Connolly, et,al.(2006) Mol. Cell |
| Gene Symbol | EIF2S1 |
|---|---|
| Official Gene Full Name | Eukaryotic translation initiation factor 2 subunit 1 |
| Alias Symbols | eIF-2-alpha; EIF-2A; EIF-2alpha; EIF2S1; Eukaryotic translation initiation factor 2 alpha subunit; Eukaryotic translation initiation factor 2 subunit 1; IF2A |
| NCBI Gene Id | 1965 |
| Protein Name | Eukaryotic translation initiation factor 2 subunit 1 |
| Swissprot Id | P05198 |
| Protein Accession # | NP_004085.1 |
| Molecular Weight | 36 kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express EIF2S1.  |
| RNA Seq | Find tissues and cell lines supported by RNA-seq analysis to express EIF2S1.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
- Reviewed Data:
- Product Protocol: EIF2S1 antibody tested with K562 in western blot (WB) (OAAF00311)
What is the species homology for "EIF2S1 Antibody (Phospho-Ser51) (OAAF00311)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
How long will it take to receive "EIF2S1 Antibody (Phospho-Ser51) (OAAF00311)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
What buffer format is "EIF2S1 Antibody (Phospho-Ser51) (OAAF00311)" provided in?
This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "EIF2S1 Antibody (Phospho-Ser51) (OAAF00311)"?
This target may also be called "eIF-2-alpha; EIF-2A; EIF-2alpha; EIF2S1; Eukaryotic translation initiation factor 2 alpha subunit; Eukaryotic translation initiation factor 2 subunit 1; IF2A" in publications.
What is the shipping cost for "EIF2S1 Antibody (Phospho-Ser51) (OAAF00311)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "EIF2S1 Antibody (Phospho-Ser51) (OAAF00311)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "EIF2S1 Antibody (Phospho-Ser51) (OAAF00311)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "36 kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "EIF2S1 Antibody (Phospho-Ser51) (OAAF00311)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "EIF2S1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "EIF2S1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "EIF2S1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "EIF2S1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "EIF2S1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "EIF2S1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
