| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Liquid. PBS with 0.09% (W/V) sodium azide. |
| Clonality | Monoclonal |
| Clone | 1486CT328.53.37 |
| Isotype | IgG1, k |
| Host | Mouse |
| Application | IHC-P, FC, WB, ELISA |
| Additional Information | Cellular Location: Cell membrane; Single-pass type I membrane protein.Tissue Location: Expressed in bone marrow and in differentiated blood mononuclear cells. |
| Reconstitution and Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Replacement Item | This antibody may replace item sc-118887 from Santa Cruz Biotechnology. |
| Immunogen | This CSF1R antibody is generated from a mouse immunized with a recombinant protein. |
| Purification | This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Concentration | 0.5 mg/ml |
| Datasheets/Manuals | Printable datasheet for anti-CSF1R (OAAB18690) antibody |
| Application Info | IHC-P: 1:25FC: 1:25WB: 1:4000 |
| Target Reference | Hampe A.,et al.Oncogene Res. 4:9-17(1989).Coussens L.,et al.Nature 320:277-280(1986).Andre C.,et al.Genomics 39:216-226(1997).Jin P.,et al.Arthritis Res. Ther. 10:R73-R73(2008).Schmutz J.,et al.Nature 431:268-274(2004). |
| Gene Symbol | CSF1R |
|---|---|
| Alias Symbols | Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms, CD115, CSF1R, FMS |
| NCBI Gene Id | 1436 |
| Description of Target | Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP- 1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. |
| Swissprot Id | P07333 |
| Molecular Weight | 108 kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express CSF1R.  |
| RNA Seq | Find tissues and cell lines supported by RNA-seq analysis to express CSF1R.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
What is the species homology for "CSF1R Antibody (OAAB18690)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
How long will it take to receive "CSF1R Antibody (OAAB18690)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
What buffer format is "CSF1R Antibody (OAAB18690)" provided in?
This item is provided in "Liquid. PBS with 0.09% (W/V) sodium azide. ".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "CSF1R Antibody (OAAB18690)"?
This target may also be called "Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms, CD115, CSF1R, FMS" in publications.
What is the shipping cost for "CSF1R Antibody (OAAB18690)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "CSF1R Antibody (OAAB18690)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "CSF1R Antibody (OAAB18690)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "108 kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "CSF1R Antibody (OAAB18690)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "CSF1R"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "CSF1R"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "CSF1R"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "CSF1R"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "CSF1R"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "CSF1R"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
