| Predicted Species Reactivity | Cow, Dog, Human, Mouse, Rat |
|---|---|
| Product Format | Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | WB |
| Additional Information | Packaging : 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
| Reconstitution and Storage | For long term storage -20C is recommended. Stable at -20C for at least 1 year. |
| Immunogen | Synthetic peptide corresponding to amino acid residues from the C-terminal region of rabphilin 3A. |
| Purification | Prepared from rabbit serum by affinity purification on a column to which the C-terminal peptide immunogen was coupled. |
| Predicted Homology Based on Immunogen Sequence | Cow; Dog; Human; Mouse; Rat |
| Datasheets/Manuals | Printable datasheet for OAPC00058 |
| Specificity | Specific for the ~82k rabphilin 3A protein. |
| Application Info | The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, human, bovine, canine and non-human primate tissue based on the fact that these species have 100% homology with the amino acid sequence used as antigen.Western blot of rat cortex lysate showing specific immunolabeling of the ~82k rabphilin 3A. |
| Target Reference | Foletti DL, Scheller RH (2001) Developmental regulation and specific brain distribution of phosphorabphilin. J Neurosci 21:5461-5472.Fykse EM, Li C, Sudhof TC (1995) Phosphorylation of rabphilin-3A by Ca2+/calmodulin- and cAMP-dependent protein kinases in vitro. J Neurosci 15:2385-2395.Geppert M, Bolshakov VY, Siegelbaum SA, Takei K, De Camilli P, Hammer RE, Südhof TC (1994) The role of Rab3A in neurotransmitter release. Nature (Lond) 369:493-497.Li C, Takei K, Geppert M, Daniell L, Stenius K, Chapman ER, Jahn R, De Camilli P, Südhof TC (1994) Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid- binding protein, depends on rab3A/3C. Neuron 13:885-898.Smith R, Petersen A, Bates GP, Brundin P, Li JY (2005) Depletion of rabphilin 3A in a transgenic mouse model (R6/1) of Huntington"s disease, a possible culprit in synaptic dysfunction. Neurobiol Dis 20:673-684. |
| Gene Symbol | RPH3A |
|---|---|
| NCBI Gene Id | 171039 |
| Protein Name | Rabphilin-3A |
| Description of Target | Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994;Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington"s disease (Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995;Foletti and Scheller, 2001). |
| Swissprot Id | P47709 |
| Protein Accession # | NP_598202.1 |
| Molecular Weight | ~82kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express RPH3A.  |
| RNA Seq | Find tissues and cell lines supported by DNA array analysis to express RPH3A.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
What is the species homology for "Anti-Rabphilin 3A (OAPC00058)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Cow, Dog, Human, Mouse, Rat".
How long will it take to receive "Anti-Rabphilin 3A (OAPC00058)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
What buffer format is "Anti-Rabphilin 3A (OAPC00058)" provided in?
This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "Anti-Rabphilin 3A (OAPC00058)"?
This target may also be called "" in publications.
What is the shipping cost for "Anti-Rabphilin 3A (OAPC00058)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "Anti-Rabphilin 3A (OAPC00058)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "Anti-Rabphilin 3A (OAPC00058)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~82kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "Anti-Rabphilin 3A (OAPC00058)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "RPH3A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "RPH3A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "RPH3A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "RPH3A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "RPH3A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "RPH3A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
